Subproject 14

Collection and analysis of organic compounds in groundwater

The objective of subproject 14 is to determine organic compounds included in the CONAMA 396 resolution in groundwater collected in the Paraopeba River basin using the techniques of gas chromatography and liquid chromatography coupled with mass spectrometry. The evaluation of groundwater quality in the Paraopeba River basin will be carried out in accordance with the sampling plan provided by the Scientific Technical Committee (CTC) consisting of 144 sampling points. The collection of samples will be carried out by a specialized company, which will be contracted for this purpose. The collection and preservation of samples will necessarily be carried out following the protocols described in the National Guide for Collection and Preservation of Samples of the National Water Agency.

This objective is divided into: 

  • Hire and monitor the company that will carry out the collection of groundwater samples to determine the organic compounds listed in CONAMA Resolution 396;
  • Analyze the samples by applying suitable preparation methodologies for the determination of compounds contemplated in CONAMA Resolution 396 by using the technique of liquid and gas chromatography coupled with sequential and high resolution mass spectrometry, using quantitative methodologies validated according to the parameters recommended in the validation guide of the INMETRO;
  • For samples where the compounds listed in CONAMA Resolution 396 are found, and at levels above the recommended levels, re-analyses will be performed;
  • Establish similarity correlations by using chemometric tools, such as PCA (Principal Component Analysis) and HCA (Hierarchical Clustering Analysis), seeking to trace similarity profiles and distribution patterns of organic compounds from groundwater in the Paraopeba River basin.

Methodological steps: 

1st Stage - Sample collection: The collection and preservation will follow the protocols described in the ANA's National Sample Collection and Preservation Guide (National Water Agency, 2011), for the purpose of determining organic compounds contained in CONAMA 396 Standard.

2nd Stage - Analysis of organic compounds: The target (or target) analyzes are centered on the analysis of a group or class of compounds, in this case, the compounds listed in CONAMA Resolution 396. Untarget (or global) analyzes can be conveniently used as a screening method, in addition to allowing the detection of analytes that are not on the list of CONAMA Resolution 396. The analytical methods described here, which make use of the extraction method called Solid Phase Extraction (SPE), of chromatography techniques gaseous or liquid coupled to mass spectrometry and target and nontarget analyses.

3rd Stage - Extraction method used in the analysis: Solid Phase Extraction (SPE)

4th Stage - Analysis by gas chromatography coupled with mass spectrometry (GC-MS and GC-MS/MS)

5th Step - Analysis by liquid chromatography coupled to mass spectrometry (UHPLC-HRMS and UHPLC-2D-MS/MS): Target Analyzes (UHPLC-2D-MS/MS) and Untarget Analyzes (UHPLC-HRMS)

6th Stage - Data Treatment: Target Analysis and Untarget Analysis

 

Meet the team!

Subproject Timeline 14

Month 1 (11/2020)
Month 2
Month 3
Month 4
Month 5
Month 6 (11/2021)

• Hiring a specialized and accredited company to carry out the collections;
• Hiring a company to audit the collected and delivered samples;
• Delivery and storage of samples in the presence of a CTC member and a contracted auditor;
• Acquisition of consumable materials to carry out the analyses;
• Team training with equipment;
• Adjustment of sample preparation conditions and instrumental analytical parameters.

• Acquisition of materials consumables for carrying out the analyses;
• Team training with equipment;
• Adjustment of sample preparation conditions and instrumental analytical parameters;
• Validation of methods and evaluation of merit parameters;
• Validation report.

• Delivery and storage of samples in the presence of a CTC member and a contracted auditor;
• Adjustment of sample preparation conditions and instrumental analytical parameters;
• Validation of methods and evaluation of merit parameters;
• Validation report;
• Partial report with the results obtained.

• Delivery and storage of samples in the presence of a CTC member and a contracted auditor;
• Validation of methods and evaluation of merit parameters;
• Analysis of samples;
• Partial report with the results obtained.

• Analysis of samples;
• Data treatment using chemometric tools.

• Sample analysis ;
• Data treatment using chemometric tools;
• Final report with all activities involved in the subproject, results obtained, discussions and conclusions, which should contain the sample map with the distribution profile of organic compounds in georeferenced groundwater of the Paraopeba river basin. /p>

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