Subproject 17+19
Determination of organic compounds in surface waters and sediments of the Paraopeba River basin
This subproject aims to qualitatively and quantitatively evaluate organic contaminants in surface water and sediments of the Paraopeba River Basin. The analytes provided for in CONAMA Resolutions 357 (surface water) and 454 (sediment), as shown in Annex 1, will be quantified in samples collected in 44 points of the Paraopeba River Basin defined in the sampling plan of the CTC of the Brumadinho-UFMG Project.
This objective is divided into:
- Determine and quantify organic compounds provided for in CONAMA Standards 357 and 454 Annex 1) in surface water and sediment samples, respectively, sent for analysis by the CTC-UFMG, using liquid chromatography techniques coupled to sequential mass spectrometry (UHPLC-2D -MS/MS) and gas chromatography coupled to mass spectrometry (GC-MS and GC-MS/MS), using quantitative methodologies validated according to the parameters recommended in the INMETRO validation guide;
- Qualitatively and, if possible, quantitatively determine the presence of toxic organic contaminants that are not included in CONAMA Standards 357 and 454, respectively, sent for analysis by the CTC-UFMG. This will be done using the technique of liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS), employing methodologies in full scan mode (screening);
- After delivering the results of the partial reports with the results obtained and receiving the georeferencing of the samples from the CTC-UFMG, compare the results obtained with the quality guide values of the CONAMA 357 and 454 Standards. Use graphics and statistical methods for data interpretation obtained, together with the results from other subprojects within the scope of the Brumadinho-UFMG Project,
- Establish similarity correlations by using chemometric tools, such as PCA (Principal Component Analysis) and HCA (Hierarchical Clustering Analysis), seeking to trace similarity profiles and distribution patterns of organic compounds in the surface waters of the Paraopeba River basin;
- Evaluate the results obtained in relation to the work carried out by the parties involved in the lawsuits (files 5000121-74.2019.8.13.0054, 5010709-36.2019.8.13.0024, 5026408- 67.2019.8.13.0024, 5044954-73.2019.8.13.0024), which are being processed before the Court of the 2nd Public Finance Court of the District of Belo Horizonte, in addition to other environmental monitoring works and scientific studies available.
Methodological steps:
1st Stage - Sample collections: sample collections will be carried out by a team selected in other public notices (Called Projeto Brumadinho-UFMG no 9-2019 and no 11-2019 and will be distributed as follows: (1) 44 surface water samples collected monthly, for 11 months, totaling 484 collections; (2) in the case of sediments, 44 samples will be collected in two distinct periods, during the validity of the notice, totaling 88 samples.
2nd Stage - Extraction methods: Solid Phase Extraction (SPE) and Headspace (HS);
3rd Step - Extraction of organic compounds from sediment samples
4th Stage - Analysis by gas chromatography coupled with mass spectrometry (GC-MS and GC-MS/MS)
5th Step - Analyzes by liquid chromatography coupled to mass spectrometry (UHPLC-HRMS and UHPLC-2D-MS/MS): Target Analyzes (UHPLC-2D-MS/MS) and Untarget Analyzes (UHPLC-HRMS)
6th Stage - Data Treatment: Target Analysis and Untarget Analysis
Meet the team!
Subproject Timeline 17+19
• Meeting with the general coordinator of CRA;
• Acquisition of consumable materials to carry out the analyses;
• Training on equipment and elaboration of Work Instructions (ITs);
• Adjustment of sample preparation conditions and instrumental analytical parameters;
• Preliminary comparative evaluation between ultrasound and accelerated solvent extraction techniques.
• Equipment training and elaboration of Work Instructions (ITs);
• Adjustment of sample preparation conditions and instrumental analytical parameters;
• Preliminary comparative evaluation between ultrasound and accelerated solvent extraction techniques;
• Validation of methods and evaluation of merit parameters.
• Delivery and storage of the collected samples;
• Start of analysis of collected samples;
• Preliminary comparative evaluation between ultrasound and accelerated solvent extraction techniques;
• Validation of methods and evaluation of merit parameters;
• Validation report.
• Delivery and storage of the collected samples;
• Preliminary comparative evaluation between ultrasound and accelerated solvent extraction techniques;
• Validation report;
• Analysis of samples.
• Delivery and storage of the collected samples;
• Analysis of samples.
• Delivery and storage of collected samples;
• Analysis of samples;
• Partial report with the results obtained.
• Delivery and storage of the collected samples;
• Analysis of samples;
• Partial report with the results obtained.
• Delivery and storage of collected samples;
• Analysis of samples.
• Delivery and storage of the collected samples;
• Analysis of samples.
• Delivery and storage of the collected samples;
• Analysis of samples.
• Delivery and storage of the collected samples;
• Analysis of samples;
• Data treatment using chemometric tools.
• Delivery and storage of the collected samples;
• Analysis of samples;
• Data treatment using chemometric tools;
• Final report with all activities involved in the subproject, results obtained, discussions and conclusions, which should contain the sample map with the distribution profile of organic compounds in georeferenced groundwater of the Paraopeba River Basin.
• Sample analysis ;
• Final report with all activities involved in the subproject, results obtained, discussions and conclusions, which should contain the sample map with the distribution profile of organic compounds in georeferenced groundwater of the Paraopeba River Basin.